Benefits of HPLC
HPLC refers to high performance liquid chromatography and it is a commonly used analysis method used in the fields of biochemistry and chemistry. It is used for identifying and separating compounds. HPLC uses complex equipment that is more advanced in contrast with other kinds of chromatography. Understanding the benefits of HPLC will assist you to appreciate the complex way the HPLC functions.
1. High definition results
HPLC produces high definition results that allow scientists to make factual theories. This is because HPLC uses a pump for producing high pressures and thus the compounds and other components separate definitively. Other methods of separation may result in unclear results since they depend mainly on gravity. This leads to low separation and it makes reading results hard. Hence, HPLC is the best separation technique to use if you desire clear results.
HPLC is a modernized tool used for separating compounds on the basis of their polarity. In consequence, it works much more quickly as compared to its predecessors. The conventional column chromatography techniques heavily relied on the force of gravity so as to separate the compounds. However, thanks to the creation of HPLC, it is now possible to quickly separate compounds through using a pressurized pump. Results can be read within a couple of minutes.
3. Allows automation
A further HPLC benefit is the simple fact that it allows both quantification and instrumentation to be easily automated. This explains why operating the HPLC machine demands training to make certain that it is correctly performed. Due to automation of the whole process, results can be arrived at quickly in contrast with using other methods.
Since the HPLC has a very complex makeup, it is very simple to get false results when it is not correctly operated. It also has a low sensitivity and this causes misreading because of the high speed of the separation process.